Product Features:
1. High-Performance High-Pressure Chromatography Pump System
- Utilizes a high-pressure constant-flow plunger pump (dual pumps, four pump heads), with each pump head independently degassing, ensuring stable flow rate and extremely low pulsation.
- Flow rate range: 0–300 mL/min (optional 0–400 mL/min), supporting scale-up from experimental to production.
- Significantly superior performance to peristaltic pump systems, offering a more stable baseline and more accurate flow rate.
2. High-Quality Gradient and Biocompatible Flow Path
- High-pressure gradient system generates smooth, repeatable, high-quality gradient curves.
- The entire system uses bio-inert, pressure-resistant tubing, balancing high pressure resistance with the safety of biological samples.
3. Multi-Detector Integration for More Comprehensive Information
- Optional full-wavelength xenon lamp light source for the UV detector, requiring no preheating.
- Simultaneous detection of four wavelengths (190–850 nm) enhances the monitoring capability for complex samples.
- Large-range, high-precision conductivity detector (with automatic temperature correction).
- Optional pH detector with temperature compensation for high detection accuracy.
4. Fully Automated Sample Injection and Liquid Flow Management
- Fully automated high-pressure sample loading valve enables sample loading/injection. / Automatic flushing switching prevents air from entering the system
- ≥4 buffer inlet switching options, supporting multi-buffered methods
- Built-in bypass and backflushing system for easy and rapid buffer replacement and system cleaning
5. Stable and reliable fraction collection system
- Fully automated fraction collection, no tube skipping, no overflow, high stability
- X-Y matrix structure (no motor at the bottom), safer than traditional rotary table systems
- Compatible with multiple tube sizes; switching is simple by changing the tube rack
- Supports multiple modes including peak collection, volumetric collection, and manual collection, avoiding cross-contamination
6. Intelligent workstation and automated operation
- Dedicated ClearFirst-3200 protein purification workstation, fully automated computer control
- Built-in method database, one-click access, lowering the operational threshold
- Supports user management, sample management, and instrument self-testing
- Full Chinese interface, conforming to domestic laboratory usage habits
7. Integrated design, adaptable to laboratory environment
- Compact integrated structure, can be directly placed in a chromatography refrigerator
- Supports autosampler for unattended operation
- Comprehensive performance and functional configuration superior to AKTA pure This series offers excellent value for money.
Instrument Components:
1. Main Unit (including high-pressure constant flow pump, injection valve, and collection valve);
2. UV Detector (including full-wavelength UV detector, flow cell, and digital-to-analog converter);
3. Conductivity Detector (including flow cell and digital-to-analog converter);
4. Pressure Sensor (standard location after pump);
5. Valve System (including buffer selection valve, bypass system valve, and backflush system valve);
6. Large-Volume Collection System;
7. Fully Automated Fraction Collector (including main unit, tube rack, and three-way solenoid valve);
8. ClearFirst-3200 Protein Purification Workstation;
Optional Components:
1. pH Detector (including flow cell and digital-to-analog converter);
2. Bubble Detectors (up to 4, before system pump/before sample pump/after sample loading valve);
3. Column Selection Valve (can accommodate 5 columns + 1 bypass);
4. Large-Volume Injection System (injection pump and injection valve);
5. Fully Automated Sampler;
6. Chromatography Cooler;
Product Parameters：
| Solvent Compatibility | Commonly used aqueous buffer solutions for biomolecule purification. |
| System Pump Flow Rate Range | 0.01-300 mL/min, 150 mL/min per pump (optional 0.01-400 mL/min, 200 mL/min per pump) |
| Pressure range | 0.01-20 MPa (200 bar, 2900 psi) at 0-50 mL/min; 0.01-10 MPa (100 bar, 1450 psi) above 100 mL/min |
| Number of buffer inlets | 4, 2 per pump |
| Wavelength range | 190-850 nm |
| Wavelength accuracy | ±1 nm |
| Wavelength repeatability | ±0.2 nm |
| Optical path | 2 mm, 5 mm (optional), 10 mm (optional) |
| Conductivity range | 0-999.9 mS/cm |
| Conductivity accuracy | ≤±0.01 mS/cm |
| Number of column positions | 1 |
| Fraction collector | 18 mm tube rack (standard), 96 positions, suitable for 18*150 mm tubes and 15 mL cell centrifuge tubes |
